Figure 3

TNF-α stimulates MAdCAM-1 expression in a dose-and time- dependent manner. A. Confluent HEC endothelial cells were incubated for 24 h with increasing concentrations of mouse TNF-α (as indicated), and MAdCAM-1 induction (measured as western blotting density), expressed as a percent of the maximum, (set as the scan density in 24 h, 20 ng/ml TNF-α treated HEC, [100%]). MAdCAM-1 Expression is TNF-α concentration dependent B. HEC were incubated with 20 ng/mL TNF-α for time periods up to 48 h (as indicated). MAdCAM-1 protein expression was time dependently increased at 12, 24 and 48 h. (Max expression [100%] in these experiments was determined as the blotting scan density seen at 48 hours). Expression at T = 0 represents MAdCAM-1 expressed by un-stimulated hepatic endothelial cells. Each value represents the mean ± s.e.; each group (n = 3). *P < 0.01, **P < 0.01 vs. TNF-α treatment.