Figure 5

Glucose Disposal, Insulin Levels and Insulin Sensitivity. TG mice failed to develop insulin resistance on a HFD. In A, B, and D, WT data is shown as open squares and TG data by closed triangles. (A) OGTT performed on fasted males prior to the start of the HFD showed no significant difference between genotypes (WT n = 7 and TG n = 8). (B) OGTT performed on fasted males following the HFD challenge showed decreased glucose excursion by TG mice (WT n = 7 and TG n = 5). (C) Serum insulin levels in fasted males measured from samples acquired pre and post HFD challenge, WT values are shown by open bars (n = 8) and TG by closed bars (n = 7). (D) Results from the insulin tolerance test performed after the HFD challenge. Insulin levels increased in response to 2 weeks of HFD challenge in WT (n = 7) but not TG (n = 5) mice. All results represent the mean ± SEM. * indicates P < 0.05, ** indicates P < 0.01 and *** indicates P < 0.001 in post-hoc analysis.