Maximum force values at 3 weeks (mean ± S.E.M.). Maximum force was significantly decreased in tissues from hindlimb unloaded (HU) animals receiving saline when compared to tissues from Amb + Sal animals (p = 0.03). The addition of IGF-I significantly improved maximum force in tissues from ambulatory healing animals when compared to tissues from ambulatory healing animals that received saline (p = 0.0002). Addition of IGF-I and GH+IGF-I in HU animals significantly increased maximum force values when compared to hindlimb unloaded animals receiving saline (p = 0.0074 and p = 0.0013, respectively). a significantly different from Sham group; b significantly different from Amb + Sal group; c significantly different from HU + Sal group.