Figure 2

Effect of purinoceptors agonists in RBC loaded with Fluo-3 AM. A) ATP (50 μM), in Ca²⁺ medium (1 mM CaCl₂). B-C) Effect of a non hydrolysable analogue of ATP – ATPγS (50 μM) and a specific P2Y agonist – α2MetATP (50 μM) respectively. D) Effect of ATP (50 μM), in a free Ca²⁺ supplemented with medium 15 mM EGTA. E) Inhibition of ATP effect by a P2 antagonist – Suramin (50 μM).