Figure 1

Typical ³¹P Nuclear Magnetic Resonance spectra of an isolated rat liver. A: After 30-min control KHB normothermic perfusion. Major resonances are assigned to (a) phosphomonoesters; (b) phosphocholine; (c) intracellular inorganic phosphate (Pi); (d) glycerol-3-phosphoryl ethanolamine and glycerol-3-phosphorylcholine; (e) nucleoside-5'-triphosphates (γNTP) and diphosphates (βNTP); (f) α-NTP and α-NDP; (g) nicotinamide adenine dinucleotide; (h) uridine-5'-diphosphoglucose; (i) βNTP. The reference (methylene-diphosphonic acid) is not shown (18.40 ppm) (number of scans = 148). B: 20 min after a perfusion of butyrate 3 mmol/L, effect (from the bottom to the top) of the simultaneous addition of IAA 0.5 mmol/L (2 min) and KCN 2.5 mmol/L (10 min) (number of scans = 60).