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Table 1 Calculation of mitochondrial ATP synthesis yield in isolated liver perfused with Krebs-Henseleit Buffer or substrates

From: Decrease in oxidative phosphorylation yield in presence of butyrate in perfused liver isolated from fed rats

 

ATP synthesis rate

VO2

ATP/O

KHB

1.09 ± 0.13

1.86 ± 0.16

0.30 ± 0.05

(n = 7)

-

-

-

Acetate

0.76 ± 0.10

1.92 ± 0.16

0.20 ± 0.02

(n = 7)

NS

NS

NS

Butyrate

0.40 ± 0.10*

2.54 ± 0.18*

0.07 ± 0.02**

(n = 7)

p = 0.001

p = 0.02

p = 0.0006

Octanoate

0.56 ± 0.10***

3.04 ± 0.15***

0.09 ± 0.018***

(n = 5)

p = 0.01

p = 0.0004

p = 0.005

  1. ATP synthesis rate and mitochondrial VO2 are expressed in μmole/min.g liver ww. ATP/O = ATP (μmol ATP)/2 VO2 (μatoms oxygen). The control group was perfused with Krebs-Henseleit buffer (KHB); the fatty acids were perfused at 3 mmol/L. Values are given as mean ± SEM (Significant ANOVA and t-test).
  2. * p < 0.03 versus acetate, ** p = 0.0006 versus acetate, *** NS versus butyrate.
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BMC Physiology

ISSN: 1472-6793

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