Decrease in oxidative phosphorylation yield in presence of butyrate in perfused liver isolated from fed rats

BMC Physiology

Table 2 Effects of various sequences of ATP synthesis inhibition in KHB group

Sequences	IAA following by KCN		KCN following by IAA		Simultaneous IAA+KCN
	*IAA*	*KCN*	*KCN*	*IAA*	*IAA+KCN*
Residual ATP content (% of the initial value)	90%	0	20–40%	0	0
Residual mitochondrial VO ₂ (% of the initial value)	110% (transient)	0	0	0	0
Initial rate of ATP hydrolysis*	ND	1.5	1.0	ND	1.0
Total inhibition of:	Glycolysis only	OP only	OP only	Glycolysis only	Glycolysis and OP simultaneously

* arbitrary units; 1.0 corresponding to the rate of ATP decrease in simultaneous IAA+KCN sequence (1.09 ± 0.13 μmol/min.g liver wet weight). OP = oxidative phosphorylation.

ISSN: 1472-6793