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Figure 1 | BMC Physiology

Figure 1

From: Epigenetic and phenotypic changes result from a continuous pre and post natal dietary exposure to phytoestrogens in an experimental population of mice

Figure 1

(a) scheme shows the distribution of CpG sites in the region analyzed for methylation changes in the promoter of Acta1 and their distance to the starting of transcription, describing also the sequence analyzed. (b) and (c) correspond to sequencing of PCR products amplified from bisulphite treated DNA that includes the 8 CpG sites analyzed, which are indicated with arrows. (b) shows electrophenograms with direct sequencing data and (c) shows raw data electrophenograms from which CpG DNA methylation was quantified; shown sequences were obtained with the reverse primer; CpG sites in the forward strand appear in the reverse strand as CG when methylated and as CA when unmethylated.

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BMC Physiology

ISSN: 1472-6793

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