Table 1 The primers used for cloning P450 genes, qRT-PCR reactions, and SNP determinations
Gene | Primer Name | Function | Primer Sequence | Position (nt) |
|---|---|---|---|---|
CYP4G2 | AP450HF20F | Full length cloning | 5' ATGGATAGTGCAAACAATTCAACTGCG 3' | 1 to 27 |
| Â | SNP450HF20F | SNaP determination | 5' AAATGGCCATTTGGTGGCCCAT 3' | 174 to 195 |
| Â | RTP450HF20F | qRT-PCR | 5' CGAGGAGGATGATGAAATAAGCAAGC 3' | 837 to 862 |
| Â | RTP450HF20R | qRT-PCR | 5' TTGGACATGGCCATCATGGCATCT 3' | 957 to 980 |
| Â | P450HF20R | 5'-RACE | 5' GCAGGAAGTTGTCACCAAAGATG 3' | 1140 to 1162 |
| Â | P450HF20F | 3'-RACE | 5' CGTGCATCGCAATCCCCAATAC 3' | 1341 to 1362 |
| Â | AP450HF20R | Full length cloning | 5' TTACATAGCTTTCATGGCTTCGGGTC 3' | 1625 to 1650 |
CYP4D4v2 | AP450HF5F | Full length cloning | 5' ATGTTATTTGAATTCCTGGTGGGTC 3' | 1 to 25 |
| Â | SNP450HF5F | SNaP determination | 5' ACATGACACCACGACAAGTGG 3' | 948 to 968 |
| Â | RTP450HF5F | qRT-PCR | 5' AGGATAAGGAGAAACCGGTGACC 3' | 1052 to 1074 |
| Â | RTP450HF5R | qRT-PCR | 5' CAATTGTCGGCACCGATGGATAC 3' | 1156 to 1134 |
| Â | P450HF5R | 5'-RACE | 5' AGCAACTCAAAATGGCGTACC 3' | 1430 to 1410 |
| Â | AP450HF5R | Full length cloning | 5' TAACTACTTGCGAACTCTCAAACCC 3' | 1521 to 1497 |
CYP6A38 | P450HF17P-F | 5' flanking region cloning | 5' TGGTCTTCTAGGGGAGAAGACTACCTGC 3' | -676 to -649 |
| Â | SNP450HF17PF | SNaP determination | 5' TTCAGGATTGCTGGGTAGCT 3' | -69 to -50 |
| Â | P450HF16F-3 | Full length cloning | 5' CATTATGGAGACTTCGGGAGTTTTG 3' | -4 to 21 |
| Â | P450HF17P | 5' flanking region cloning | 5' CGTAGGTTCCTCATGGGGTATACCCAGC 3' | 120 to 93 |
| Â | RTP450HF17F | qRT-PCR | 5' CCCTGATGGGCAACATGAATGGAT 3' | 122 to 145 |
| Â | RTP450HF17R | qRT-PCR | 5' TAGTTGTTTGTCCAGCAGCACCAC 3' | 270 to 247 |
| Â | P450HF17R | 5' RACE | 5' CGCTGTACTTCAATAGATTTCCTGC 3' | 650 to 626 |
| Â | P450HF17F | 3' RACE | 5' TGAGGGTGATACAAAACCAAGC 3' | 1017 to 1038 |
| Â | AP450HF17R | Full length cloning | 5' GAGATAATCTCCCACCCCTAAATCG 3' | 1520 to 1496 |
Common | Flyh1 | Â | 5' GGICCIAGIAACTGCATIGG 3' | Â |
| Â | Flyc1 | Â | 5' GGAAGTNGACACNTTYATGTT 3' | Â |
| Â | CYP6AD1 | Â | 5' GTNATHGGHHNBTGYGCHTTYGG 3' | Â |
| Â | HemeR1 | Â | 5' CCIATGCAGTTICTIGGICC 3' | Â |
| Â | Oligo (dt) | Â | 5' TAATACGACTCACTATAGGGAGATTTTTTTTTTTTTTTT 3' | Â |
| Â | C2 | Â | 5' TAATACGACTCACTATAGGGAGA 3' | Â |
| Â | AP1 (RACE) | Â | 5' CCATCCTAATACGACTCACTATAGGGC 3' | Â |
| Â | AP1 (GenomeWalking) | Â | 5' GTAATACGACTCACTATAGGGC 3' | Â |
| Â | ActinS1 | Â | 5' AGGCGAATCGCGAGAAGATG 3' | Â |
| Â | ActinAS1 | Â | 5' TCAGATCACGACCAGCCAGATC 3' | Â |