Figure 3

Pharmacological characterization of z-Shal3 currents. A and B: Effect of 4-aminopyridine (4-AP, 10 mM)(A) and flecanide (300 μM) on zShal3 (left) and Kv4.3 currents (right). C and D: Effects of PKC activation on zShal3 currents. zShal3 currents before and 30 min after exposure to 10 nM PMA (C). Currents were elicited by 900 ms voltage steps from a holding potential of -120 mV to the test potential at +50 mV. Time course of the effect of PMA (10 nM) on zShal currents (D). The peak current amplitude was normalized to the current amplitude before PMA application (time = 0). Results are expressed as mean ± SEM of 8 experiments in each group.