Figure 5

DFMO prevents actions of amino acid but not of polyamines. Hypoxic flies were fed on a 10% sucrose diet supplemented with 10 mM of the indicated compounds and survivorship curves were determined. Experiments were performed in the absence or the presence of 10 mM DFMO. Percentage changes in longevity by DFMO are indicated for each dietary condition. A log rank test was used to compare survivorship curves. **: p < 0.0001, no asterisk: p > 0.001. A. Control conditions. Curves on the left are survivorship curves obtained under starvation conditions in the absence (dots, n = 50) or the presence (curve, n = 80) of 10 mM DFMO. Curves on the right are survivorship curves obtained with a 10% sucrose medium in the absence (dots, n = 237) or the presence (curve, n = 118) of 10 mM DFMO. B-F. 10% sucrose diet supplemented with 0.1% casein or 10 mM amino acid or polyamine as indicated. Experiments were performed in the absence (magenta) or the presence of 10 mM DFMO (blue). Sample sizes were B (control, 189; DFMO, 48), C (control, 130; DFMO, 88), D (control, 208; DFMO, 59), E (control, 146; DFMO, 48) and F (control, 110; DFMO, 60).