Figure 3
Recovery from 30 min exposure to 10 mM sodium azide (NaN 3 ). A. L3 stage worms (PE255), B. Young adults (PE255), C. Gravid adults (PE255). Luminescence (in vivo) increased rapidly from the time NaN3 was removed and reached the level of worms exposed to 0 mM NaN3 within 30 min. This fast recovery is consistent with recovery in ATP production, following removal of the respiratory inhibitor NaN3. Luminescence was expressed as a % of controls exposed to 0 mM NaN3. At each time point, the luminescence of independent samples was read following 3 min incubation with luciferin. Error bars show the SEM. The pooled number of worms was 2400 (± 198) per time point. D. In vitro ATP levels of N2 worms before, after 30 min exposure to 10 mM NaN3, and after 30 min recovery from 30 min exposure to 10 mM NaN3. Azide caused a significant (**, ANOVA, P < 0.001) reduction in the levels of in vitro ATP normalised to protein content. These levels returned to the levels of worms exposed to 0 mM NaN3 within 30 min of washing worms from the chemical (ANOVA, P > 0.05). Error bars show the SEM. The pooled number of worms was 4000 (± 429) per tested condition.