Figure 5
From: Short-term pacing in the mouse alters cardiac expression of connexin43
Alterations in Cx43 Distribution and Ubiquitination with Pacing. A) Fractionation of heart samples suggests that Cx43 is reduced in the sarcolemma-enriched fraction of the paced hearts and increased in the non-sarcolemmal supernatant. B) Immunoprecipitation for Cx43 and blotting for ubiquitin suggests that there is an increased accumulation of ubiquitinated Cx43 in the paced hearts compared to sham-paced controls. In this image, mono-ubiquitinated Cx43 is seen just above the 40 kD size marker and poly-ubiquitinated forms of Cx43 appear as a slower migrating smear.