Figure 3

(A) IFN-γ decreases PECAM-1 transcript level and elevates the ICAM-1 transcript level in liver SECs or MNPs. Northern blot analysis of total RNA extracted from primary cultures of SECs or MNPs at 24 h after isolation either untreated (0 h) or treated with IFN-γ (1000 (10³) or 10000 (10⁴) U/ml) for 12 h. Five μg of total RNA was separated on agarose gel, blotted and hybridised with a ³²P-dCTP-labeled cDNA probe specific for rat PECAM-1. Same membranes were hybridised with a cDNA specific for rat ICAM-1 or for β- and γ-actin. This figure shows results of five experiments from five different isolations. The lower panel shows a statistical evaluation of densitometric scans. Error bars indicate standard deviation of the mean value after normalization on β,γ-actin expression. The P-value was tested to show significant differences of control cells compared to cells treated with IFN-γ. * indicates p < 0.05. (B) Northern blot analysis of total RNA extracted from primary cultures of SECs at day 1 after isolation treated with IFN-γ (1000 U/ml) for 4 h, 8 h, 12 h or 24 h. Five μg of total RNA was separated on agarose gel, blotted and hybridised with a ³²P-dCTP-labeled cDNA probe specific for rat PECAM-1. The same membranes were hybridised with a cDNA specific for rat ICAM-1 or for β- and γ-actin. (c) The untreated control. The lower panel shows a statistical evaluation of densitometric scans. Error bars indicate standard deviation of the mean value normalized on β,γ-actin expression of five experiments from five different isolations of each cell type. P-value was tested to show significant differences of control cells compared to cells treated with IFN-γ. * indicates p < 0.05.