Figure 5

TNF-β reverses the effect of IFN-γ on PECAM-1 transcript level but has no additional effect on the ICAM-1 transcript level in liver SECs and MNPs. Northern blot analysis of total RNA extracted from primary cultures of SECs and MNPs at day 1 after isolation either untreated (c) or treated with IFN-γ 10000 (10⁴)U/ml (I) or IFN-γ together with TNF-β (10 ng/ml) (I+T) for 12 h. Five μg of total RNA was separated on agarose gel, blotted and hybridised with a ³²P-dCTP-labeled cDNA probe specific for rat PECAM-1. The same membranes were hybridised with a cDNA specific for rat ICAM-1 or for β- and γ-actin. This figure shows results of five experiments from five different isolations. The lower panel shows a statistical evaluation of densitometric scans. Error bars indicate standard deviation of the mean value after normalization on β,γ-actin expression. P-value was tested to show significant differences of control cells compared to cells treated with IFN-γ or IFN-γ +TNF-β. * indicates p < 0.05.