Figure 7
(A) HE staining of liver sections of C57BL/6 mice (a, c, e, g, i) and B6. 129S7-Ifngtm1Ts (IFN-γ-/-) mice (b, d, f, h, k). Sections of normal livers (a, b) or acutely damaged livers (after a single CCl4-administration) at time points 12 h (c, d), 24 h (e, f), 48 h (g, h) and 72 h (i, k) are given. (B) Lack of decrease of PECAM-1 specific transcript level in CCl4-treated IFN-γ-/- mice livers and reduced increase of the ICAM-1 specific transcript level. C57BL/6 mice (controls) and B6.129S7-Ifngtm1Ts (IFN-γ-/-) mice were treated with CCl4 and livers were taken for analysis 3 h (3 h), 6 h (6 h) or 12 h (12 h) after treatment (each time point two animals). (c) The untreated control. 10 μg of total RNA was separated on agarose gel, blotted and hybridised with a 32P-labeled cDNA probe specific for mouse PECAM-1. β,γ-actin was used as loading control. This figure shows results for 2 experiments of two animals sacrificed at each time point.