Late pupa of the flesh fly Calliphora vicinia (Diptera). Fixed in hot ethanol and stained with PTA. Pupae must be perforated for PTA to penetrate. Top: volume rendering with a horizontal cutaway. Bottom: parasagittal and horizontal single reconstructed sections. Scale bar: 1 mm. Samples prepared and provided by Marlis Forcher, Univ. Vienna. SkyScan 1174 scan, voxel size 7.7 μm.