Figure 2
From: Coral bleaching under thermal stress: putative involvement of host/symbiont recognition mechanisms
Sequence and structure analysis of PdC-Lectin gene and protein. A) cDNA and derived amino acid sequence of PdC-Lectin. Black boxes indicate a predicted signal peptide. Underlined area indicates the sequence span of an identified C-type lectin domain. Grey boxes denote conserved mannose binding motifs in a Ca2+-dependent manner. Cysteines belonging to the C-type lectin conserved array are in bold. The WIGL motif is highlighted in grey. B) the alignment of C-type lectin domains of PdC-lectin with different C-type lectin domains contained in several similar proteins identified by Blast searches. Conserved amino acid positions are highlighted. Accession numbers: Millectin (GenBank: EU717895), human CD23 (GenBank: P06734), human DC-SIGN (GenBank: Q9NNX6), Mermaid-1 (GenBank: AY927371). C) the 3D structure of PdC-Lectin CRD. Crystal structure of DC-SIGNR CRD (yellow) and molecular model of PdC-Lectin CRD (green). Superposition of the crystal structure of DC-SIGNR CRD and molecular model of PdC-Lectin CRD (C). All conserved motifs are highlighted; "WIGL" is in clear blue, "EPN" in green, "WND" in pink. The four highly conserved cysteines are in dark blue.