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Figure 1 | BMC Physiology

Figure 1

From: Manipulating insulin signaling to enhance mosquito reproduction

Figure 1

Protein expression of AaegPTEN3 & 6 in female tissues: AaegPTEN3 and 6 expression in various female tissues was detected using immunoblot analysis. Tissues from 3–5 day old female mosquitoes were dissected in 10× protease inhibitor (PI) buffer, size fractioned on 12% SDS-PAGE gels, transferred to PVDF membrane and probed with anti-AaegPTEN3 or 6 antibodies. A. AaegPTEN3 and 6 recombinant proteins were immunoblotted and each were probed with the AaegPTEN3 or 6 antibodies. Both the AaegPTEN3 and 6 antibodies were specific to the appropriate recombinant protein and did not recognize the other splice variant. B. AaegPTEN6 was detected in all tissues from adult females examined, including non-bloodfed heads (HD), thoraxes (TH), fat bodies (FB), midguts (MG) and ovaries (OV). AaegPTEN3 could only be detected in the head. C. AaegPTEN6 was expressed in early embryos, pupae and adults. However, it was found at very low levels in diapausing eggs and not at all during larval stages.

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