Figure 3

Generation of dsRNA constructs against AaegPTEN and two key splice variants: Three dsRNA constructs were synthesized against AaegPTEN. Universal AaegPTEN dsRNA was generated against the identical PTEN region and was capable of knocking down all AaegPTEN splice variants. The AaegPTEN3 dsRNA was generated against ~300 bp of its unique 3' terminus and should only silence the AaegPTEN3 splice variant. AaegPTEN6 dsRNA was generated against the unique 18 bp coding sequence and ~300 bp of the 3' UTR and should only silence the AaegPTEN6 splice variant. dsRNA was also generated against ~300 bp of DsRed as a control (not shown).