Both ischemic preconditioning and ghrelin administration protect hippocampus from ischemia/reperfusion and upregulate uncoupling protein-2

Table 1 Comparison of numbers of surviving CA1 neurons and relative amounts of hippocampal UCP-2 mRNA determined in the various groups.

	----------IPC experiment ----------				---Ghrelin experiment---
GROUP	1)CON	2)I/R	3)IPC	4)IPC+I/R	5)CON	6)I/R	7)I/R+G
Number of surviving neurons/mm (n = 4/6)	231.6 ± 5.2	41.1^a ± 7.7	237.8 ± 12.1	194.1 ± 9.8	251.3 ± 8.7	28.5 ^a ± 7.3	179.5 ^{b, c} ± 11.5
Relative amount (%) of UCP-2 mRNA (n = 6/4)	0.31 ± 0.03	0.44^b ± 0.04	1.45^a ± 0.02	0.46^b ± 0.04	0.32 ± 0.02	0.46^b ± 0.03	1.16^a ± 0.04
Day of sacrifice	D3	D3	D3	D6	D3	D3	D3

mRNA expressed relative to that of β-actin. Day of sacrifice with respect to the first ischemia or sham ischemia (D0). Left 4 columns are set of measurements from IPC experiments; right 3 columns are set of measurements from ghrelin experiments. Bold characters: Values taken from Liu et al [34]. a: significantly different from all other groups, P < 0.001. b: significantly different from CON, P < 0.05. c: significantly different from I/R, P < 0.001. ANOVA + Tukey's test.

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