Figure 2

AQP6 protein expression in rat gastrointestinal tract and brush border membrane vesicles (BBMV). a Anti-AQP6 immunoblot of membranes isolated from X. laevis oocytes injected with AQP6 cRNA and non-injected oocytes (control). Two bands of about 55 and 28 kDa were observed. b Immunoblot of membrane fractions from rat stomach and kidney probed with anti-AQP6 preadsorbed with the immunizing peptide, c, probed with anti-AQP6 antibody or, d, probed with pre-immune rabbit serum. e Anti-AQP6 immunoblot of membranes from different portions of the rat gastrointestinal tract (left) and of BBMV from rat small intestine (right). A major band of about 55 kDa was observed (AQP6 dimer). f Immunoblot of jejunal homogenates incubated with (ENDO H +) or without (ENDO H -) endoglycosidase Hf. Bands of 55 kDa (AQP6 dimer), 36 kDa (glycosylated form) and 28 kDa (nonglycosylated form) were shown. MW, molecular weight markers.