Figure 3

In situ hybridization analysis of AQP6 mRNA expression. The probe detects intercalated cells of the collecting ducts in rat kidney (b). AQP6 mRNA was detected in cells located in the isthmus, neck and basal region of stomach glands, while pit cells were almost negative (d). In jejunum, the probe labelled both crypt and villus epithelial cells (f). A similar staining was detected in the colon where labelling is visible along the whole crypt (h). No specific signal was detectable in negative control sections from each tissue (a, c, e and g). Bars correspond to 100 μm.