Analysis of knockout mice suggests a role for VGF in the control of fat storage and energy expenditure

BMC Physiology

Table 2 Expression of genes in epididymal WAT and interscapular BAT that contribute to lipolysis or lipogenesis in VGF knockout and wild type mice.

Tissue	Gene	Mean Cycle Threshold (C_t) Value	Std. Error	Fold Difference in mRNA Levels (2^ΔΔCt)	P-Value	RNA Levels in VGF_C57 -/- vs +/+ mice
BAT	ACC	Vgf-/Vgf- 2.57 Vgf+/Vgf+ 1.52	.596 .205	2.1	NS
BAT	DGAT	Vgf-/Vgf- 3.45 Vgf+/Vgf+ 3.52	.210 .092	1.0	NS
BAT	GPAT	Vgf-/Vgf- 6.95 Vgf+/Vgf+ 8.12	.253 .127	2.3	.0060	Increased
BAT	PPARγ	Vgf-/Vgf -.633 Vgf+/Vgf+ 1.73	.285 .239	2.1	.002	Increased
WAT	ACC	Vgf-/Vgf- 5.20 Vgf+/Vgf+ 3.39	.517 .333	3.5	.001	Decreased
WAT	DGAT	Vgf-/Vgf- 6.88 Vgf+/Vgf+ 7.88	.370 .525	2.0	NS
WAT	GPAT	Vgf-/Vgf- 7.83 Vgf+/Vgf+ 9.01	.458 1.13	2.2	NS
WAT	PPARγ	Vgf-/Vgf- 4.75 Vgf+/Vgf+ 3.60	.315 .138	2.2	.010	Decreased
WAT	FAS	Vgf-/Vgf- 2.45 Vgf+/Vgf+ 4.80	.193 .227	5.1	.0098	Increased
WAT	SREBP1C	Vgf-/Vgf- 12.6 Vgf+/Vgf+ 8.99	.953 .560	12.1	.001	Decreased
WAT	HSL	Vgf-/Vgf- 7.79 Vgf+/Vgf+ 11.6	.405 1.10	14.0	.022	Increased

Real time RT-PCR analysis was carried out in quadruplicate as described in the Methods. The fold difference was calculated from the indicated mean cycle threshold (C_t) values, the amplification cycle when product can first be detected: lower C_t values correspond to greater abundance. C_t values were normalized to the expression of the housekeeping gene beta-actin (ΔΔCt), and fold difference in mRNA calculated as 2^ΔΔCt, as detailed in Methods, with p ≤ 0.05 considered significant.

ISSN: 1472-6793