Analysis of knockout mice suggests a role for VGF in the control of fat storage and energy expenditure

BMC Physiology

Table 3 Expression of genes in epididymal WAT and interscapular BAT that contribute to fatty acid oxidation in VGF knockout and wild type mice.

Tissue	Gene	Mean Cycle Threshold (C_t) Value	Std. Error	Fold Difference in mRNA Levels (2^ΔΔCt)	P-Value	RNA Levels in VGF_C57 -/- vs +/+ mice
BAT	PPARα	Vgf-/Vgf- 5.23 Vgf+/Vgf+ 5.83	.433 .222	1.5	NS
BAT	LPL	Vgf-/Vgf- 2.33 Vgf+/Vgf+ 3.48	.259 .263	2.2	.021	Increased
BAT	CPT-1	Vgf-/Vgf- 13.9 Vgf+/Vgf+ 15.3	.546 .312	2.6	.05	Increased
WAT	PGC-1	Vgf-/Vgf- 10.1 Vgf+/Vgf+ 12.2	.431 .271	4.3	.0028	Increased
WAT	COX II	Vgf-/Vgf- 1.91 Vgf+/Vgf+ 2.67	.207 .048	1.7	.019	Increased
WAT	PPARα	Vgf-/Vgf- 11.9 Vgf+/Vgf+ 14.3	.494. .112	5.3	.0069	Increased
WAT	LPL	Vgf-/Vgf- 4.09 Vgf+/Vgf+ 0.96	1.13 .111	2.0	.0232	Decreased
WAT	CPT-1	Vgf-/Vgf- 16.3 Vgf+/Vgf+ 16.7	.543 .427	1.3	NS

Real time RT-PCR analysis was carried out in quadruplicate as described in the Methods. The fold difference was calculated from the indicated mean cycle threshold (C_t) values, the amplification cycle when product can first be detected: lower C_t values correspond to greater abundance. C_t values were normalized to the expression of the housekeeping gene beta-actin (ΔΔCt), and fold difference in mRNA calculated as 2^ΔΔCt, as detailed in Methods, with p ≤ 0.05 considered significant.

ISSN: 1472-6793