Figure 2

Inhibition of heterotrimeric G _o signaling reversibly reduces the amplitude of ethyl acetate evoked EAG responses. For all fly strains, a 10^-4 dilution of ethyl acetate was used to evoke EAG responses. (A) EAG responses from Gal80^ts20/+; UAS-PTX/+ and Or83b-Gal4/+ control strains do not decrease (p > 0.5) at 32°C compared to 18°C. Gal80^ts20/Or83b-Gal4; UAS-PTX/+ flies have a significantly (p < 0.0001) higher EAG amplitude in the absence of PTX expression before heat induction (18°C) or after recovery from heat induction (18°C#) than in the presence of PTX expression (32°C). (B) EAG responses from Or83b-Gal4; UAS-rtTA/Tet^o-PTX flies are significantly (p < 0.0001) higher in the absence of PTX expression (dox -) than in the presence of PTX expression (dox +). EAG responses from flies that express PTX-insensitive G_o (PiG_o) in ORNs (Tet^o-PTX, UAS-PiG_o/+; Or83b-Gal4, UAS-rtTA) are not different (p > 0.7) whether PTX expression in ORNs is induced (dox +) or uninduced (dox -). For each genotype and treatment, at least 12 EAG recordings from minimum 6 flies were analyzed. Asterisks denote a significant (p < 0.05) change. All values are mean ± S.E.M.