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Figure 1 | BMC Physiology

Figure 1

From: Angiotensin II directly regulates intestinal epithelial NHE3 in Caco2BBE cells

Figure 1

Angiotensin II increases NHE3 but not NHE2 in Caco2BBE cells. (A) Monolayers were treated basolaterally with (A) 1 nM AII for varying times or (B) varying concentrations of AII for 48 hours. Apical NHE2 and NHE3 activities were measured as described in Materials and Methods. Apical surface NHE3 and NHE2 were measured using apical surface biotinylation. Total NHE expression was measured in whole lysates. Fluxes and images are representative of four separate experiments. Flux values are means ± SEM. Densitometric values were calculated setting intensity for the zero time value (A) or no addition (B) to 100% in each experiment and calculating densitometric changes (decreases or increases) of other groups relative to these values. * P < 0.05 + P < 0.01 compared with untreated zero time control by analysis of variance using a Bonferroni correction.

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