Figure 3

Peroxide equivalents, NO _x ^-levels, and effect of antioxidant treatment on mortality. (A) Plasma peroxide equivalents for WT and MK2^-/- mice, 2.5 h after i.v. injection of 125μg/kg TNF. Pooled data from 2 separate experiments is shown (n^PBS (WT and MK2^-/-)=13, n^TNF (WT)=11, n^TNF (MK2^-/-)=12). (B) Plasma NO_x^- levels for WT and MK2^-/- mice, 2.5 h after i.v. injection of 125μg/kg TNF. Pooled data from 2 separate experiments is shown (n^PBS (WT and MK2^-/-)=12, n^TNF (WT)=7, n^TNF (MK2^-/-)=9). Comparisons were made between baseline (PBS) and TNF challenged animals (#), and between genotypes (*) via one-way ANOVA with Sidak’s multiple comparisons test. (C) Mortality for TNF challenged MK2^-/- mice after pre- and post-treatment with tempol or SOD. Survival curves of different treatment groups were compared to controls via log-rank test (*). Error bars indicate SD. ****, p≤0.0001; ***, p≤0.001; **, p≤0.01; *, p≤0.05.