Figure 3
The effect of maternal fat supplementation on offspring intramyofibre lipid deposition. (A) Bar graph illustrating quantitative analysis of oil red O staining of intramyofibre lipid deposition in 7-day-old offspring exposed control (white; n = 7) and high fat (black; n = 7) piglets (means ± SEM (# p < 0.05)). Histological images of the biceps femoris fibres generated by oil red O staining (B: control; C: HF group) and anti-slow MyHC staining (D: control; E: HF group). Circle outlines area of interest for analysis, arrows indicate the lipid stain in relation to type I fibres. Scale bar = 50 μm.