α/β CaMKII co-overexpression in soleus and gastrocnemius muscle. A) Immunoblot of homogenate from a control-transfected (1,3) and α/β CaMKII-transfected (2,4) GM (1,2) and SOL (3,4) and muscle pairs after detection with antibody against pan-CaMKII. B, C) Immunoblot with pan-CaMKII (B) and pThr287-CaMKII antibody (C) of in vitro kinase reactions of homogenates from a α/β CaMKII-plasmid injected and porated soleus muscle after incubation in conditions: 1) suppressing (EGTA) and 2) allowing (2, Ca2+/CaM) calcium/calmodulin dependent autophosphorylation. Loading controls of the respective Ponceau S-stained membrane visualizing the skeletal α actin band are shown below. Arrows indicate the position of the bands corresponding to Calcium/Calmodulin-inducible kinase II isoforms. α and β correspond to the overexpressed CaMKII isoforms. D, E) Bar graph showing the mean + SE of α/β CaMKII protein content in control-porated (light grey filled bar) and porated and α/β CaMKII co-transfected (light grey filled bar) soleus (D) and gastrocnemius medialis (E) muscle. +, 0.05 ≤ p <0.10 vs. control-porated muscle, respectively. n = 4.