Figure 6

Phosphorylation of overexpressed CaMKII after isometric exercise in situ. A) Example of immunodetected CaMKII (top panel) and pThr287-CaMKII (bottom) in transfected GM and SOL muscle after 2-minutes of isometric exercise in situ. Endogenous and exogenous (bold font) CaMKII isoforms are labelled. Red arrows indicate threonine 287 phosphorylated β-CaMKII in lanes loaded with homogenate from stimulated samples (stim) compared to non-stimulated samples (rest). B, C) Microscopic field of α/β CaMKII-transfected SOL muscle after staining for CaMKII (red, B) and pThr287-CaMKII (blue, C). D, E) Microscopic field of α/β CaMKII-transfected GM muscle after staining for CaMKII (red, D) and pThr287-CaMKII (E). Note the congruency of CaMKII and pThr287-CaMKII stained muscle fibres. Scale bar denotes 250 μm.