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Figure 4 | BMC Physiology

Figure 4

From: Differential role of STIM1 and STIM2 during transient inward (Tin) current generation and the maturation process in the Xenopus oocyte

Figure 4

Specific STIM knockdown by oocyte injection of as-STIM differentially decreased the T in current. A) Oocytes induced to express M1, P2Y8, or P2Y2 receptors were stimulated with either ACh or ATP (100μM), and LPAR in native oocytes were stimulated by FBS (1:1000 dilution); the resulting Tin currents (CNT, gray areas) were compared with the Tin obtained in oocytes from the corresponding group that were also injected with 50 ng as-STIM1 (superimposed black traces); all responses were monitored 48-72 h after oocyte injection. B) The graph shows the results obtained using the different experimental conditions illustrated in A). C) In a set of experiments similar to those shown in A), Tin currents were monitored, and the peak amplitudes of non-injected CNT oocytes were compared with those of oocytes injected (48-72 h before recording) with 50 ng as-STIM2 and stimulated with the agonists. D) The graph shows the results obtained using the different experimental conditions illustrated in C). Bars correspond to the mean (± SEM) of the Tin peak amplitude of 10-15 oocytes from 5-6 frogs (*p<0.01, as-STIM vs. CNT).

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