Figure 5
Oocyte injection with COOH-STIM2 antibody produced a strong potentiation of T in current response. A) Tin current responses were monitored in two conditions: non-loaded oocytes (CNT) and oocytes loaded with COOH-STIM2 antibody (ab-loaded). Tin responses were elicited by ACh, FBS, or ATP application, depending on the receptor to be stimulated. In all cases, a strong potentiation of the response was observed in ab-loaded oocytes. B) Oocytes stimulated by ACh (M1) loaded with denatured COOH-STIM2 had control-like responses, while NH-STIM2 or NH-STIM1 loading did not produce Tin potentiation. C) The graph shows the results obtained using the different experimental conditions illustrated in A and B; each bar corresponds to the mean (± SEM) of the Tin peak amplitude normalized against the CNT current of 10-15 oocytes from 3-6 frogs (*p<0.01).