Detection of Kirrel3 splice variants with primer set 1. A – PCR amplicons obtained with Kirrel3 primer set 1 to detect Kirrel3 A. mRNA was isolated from adult human skeletal muscle at rest and 4 hr and 24 hr post performance of a plyometric jumping (PLYO) exercise (S = subject) and culture human astrocytes. *Note - the unlabelled lane came from subject 1 baseline, however, its GAPDH level was very low and hence, it has not been commented on in this manuscript. B – Results obtained using a nested PCR approach with Kirrel3 primer set 1 and 2 on biopsy samples from subject 2 from Figure A. C – Additional PCR amplicon (500 nucleotides) was detected using Kirrel3 Primer set 1 from a subset of adult human skeletal muscle biopsies that were obtained from participants one and two days post a downhill run (DHR) exercise bout. D – Schematic of the exon structure of amplicons detected in A and B after excision of the bands from the gels and nucleotide sequencing.