Table 1 Inter-calibration of pixel intensity (grayscales) to account for scanning artifacts detected (see Methods); X represents the raw measurements for grayscales and the calibration equation was applied to correct scanning artifacts.
Scan | Timing | Air | Brain | Range | Average air | Average range: brain-air | Calibration equation |
|---|---|---|---|---|---|---|---|
N (control) | 11988 | 21532 | 9543 | 13192 | 8635 | X *8635/9543 + 13192-11988* 8635/9543 | |
A | Day 5 | 13415 | 21113 | 7699 | (N, A, and B) | X *8635/7699 + 13192-13415* 8635/7699 | |
B | Day 10 | 14174 | 22835 | 8661 | X *8635/8661 + 13192-14174* 8635/8661 | ||
C | Day 34 | 14335 | 23019 | 8685 | 15206 | 8278 | X *8278/8685 + 15206-14335* 8278/8685 |
D | Day 49 | 16078 | 23950 | 7872 | (C and D) | X *8278/7872 + 15206-16078* 8278/7872 |