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Fig. 12 | BMC Physiology

Fig. 12

From: Subcellular dissemination of prothymosin alpha at normal physiology: immunohistochemical vis-a-vis western blotting perspective

Fig. 12

Cellular distribution of PTMA in rat primary neurons. Fixed rat primary cortical neuron cells were permeabilized with 50 and 100 % methanol then washed with PBS. The permeabilized cells were incubated in blocking buffer, and then with AntiCT (a)i and 2 F11(b)ii. Stained cells were then processed for fluorescence microscopy. C-terminal antibody stained PTMA in the primary cortical neurons with a punctate pattern while anti N-terminal showed diffuse nuclear staining. Arrowheads denote the weak cytoplasmic signals detected by the anti C-terminal PTMA antibody only

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