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Fig. 6 | BMC Physiology

Fig. 6

From: Subcellular dissemination of prothymosin alpha at normal physiology: immunohistochemical vis-a-vis western blotting perspective

Fig. 6

Immunohistochemistry analysis of PTMA in (a) mouse brain hippocampus tissue and (b) Lung tissue. Whole animal vascular perfusion fixation was conducted with PFA and the required tissues were isolated. The brain hippocampus and lung were processed for immunohistochemistry by embedding to paraffin wax. Tissue sections were stained with AntiCT (a)i and (b)i and 2 F11 (a)ii and (b)ii together with relevant fluorescent tagged antibodies. Sections were then washed, counterstained with Hoechst (a)iii and (b)iii and processed for fluorescence microscopy. Both antibodies localized PTMA in the nucleus of the hippocampus (a)i and ii, while in the lung PTMA immunoreactivity was observed in the nucleus and cytoplasm by AntiCT (b)i but mainly in the cytoplasm by 2 F11 (b)ii

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BMC Physiology

ISSN: 1472-6793

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