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Fig. 7 | BMC Physiology

Fig. 7

From: Subcellular dissemination of prothymosin alpha at normal physiology: immunohistochemical vis-a-vis western blotting perspective

Fig. 7

Immunohistochemical examination of PTMA in (a) mouse heart and (b) kidney tissue. Whole animal vascular perfusion fixation was conducted with PFA and the required tissues were isolated. The heart and kidney were processed for immunohistochemistry by embedding to paraffin wax. Tissue sections were stained with AntiCT (a)i and (b)i and 2 F11(a)ii and(b)ii together with relevant fluorescent tagged antibodies. Sections were then washed, counterstained with Hoechst (a)iii and(b)iii) and processed for fluorescence microscopy. The heart PTMA immunoreactivity was moderate by both antibodies (a)i and ii. The kidney AntiCT showed higher immunofluorescent intensity in comparison to 2 F11 signals in the Kidney (b)i and ii

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BMC Physiology

ISSN: 1472-6793

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