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Fig. 8 | BMC Physiology

Fig. 8

From: Subcellular dissemination of prothymosin alpha at normal physiology: immunohistochemical vis-a-vis western blotting perspective

Fig. 8

Immunohistochemical examination of PTMA expression levels in (a) mouse liver tissue and (b) stomach. Whole animal vascular perfusion fixation was conducted with PFA and the required tissues were isolated. The liver and stomach were processed for immunohistochemistry by embedding to paraffin wax. Tissue sections were stained with AntiCT (a)i and (b)i and 2 F11 (a)ii and (b)ii together with relevant fluorescent tagged antibodies. Sections were then washed, counterstained with Hoechst (a)iii and (b)iii and processed for fluorescence microscopy. The liver shows substantial nucleic PTMA immunoreactivity by both antibodies (a)i and ii whereas only the cytoplasm showed immunofluorescence by both 2 F11 and AntiCT in the stomach (b)i and ii

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BMC Physiology

ISSN: 1472-6793

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