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Fig. 9 | BMC Physiology

Fig. 9

From: Subcellular dissemination of prothymosin alpha at normal physiology: immunohistochemical vis-a-vis western blotting perspective

Fig. 9

Immunohistochemical analysis of PTMA in (a) mouse duodenum and (b) Jejunum. Whole animal vascular perfusion fixation was conducted with PFA and the required tissues were isolated. The duodenum and jejunum were processed for immunohistochemistry by embedding to paraffin wax. Tissue sections were stained with AntiCT (a)i and (b)i and 2 F11 (a)ii and (b)ii together with relevant fluorescent tagged antibodies. Sections were then washed, counterstained with Hoechst (a)iii and (b)iii) and processed for fluorescence microscopy. In the duodenum, PTMA immunoreactivity revealed nucleic staining with AntiCT (b)i and cytoplasmic staining with 2 F11 (b)ii

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BMC Physiology

ISSN: 1472-6793

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