Fig. 3From: Ryanodine-induced vasoconstriction of the gerbil spiral modiolar artery depends on the Ca2+ sensitivity but not on Ca2+ sparks or BK channelsInhibition of BK channels with iberiotoxin or inhibition of Ca2+ sparks with tetracaine does not increase the global [Ca2+]i or constrict the SMA at 40 cmH2O. a Representative recordings of [Ca2+]i changes from single smooth muscle cells from a pressurized (40 cmH2O) SMA in response to 100 nM Ibtx. b Average of normalized traces of [Ca2+]i changes in the presence of Ibtx (65 cells). Traces in a and b were normalized as described in Methods. c Average trace of corresponding changes in vascular diameter in the presence of Ibtx (8 arteries). d Representative recordings of [Ca2+]i changes from single smooth muscle cells from a pressurized (40 cmH2O) SMA in response to 100 μM Tet and 1 μM nifedipine (Nif). e Average of normalized traces of [Ca2+]i changes at 40 cmH2O (26 cells). Traces in d and e were normalized as described in Methods. f Average trace of corresponding changes in vascular diameter (5 arteries) in the presence of Tet and Nif. Diameter changes were normalized to the average of values recorded between 30–60s (value indicated by ‘a’ was set to 1). [Ca2+]i and diameter data were simultaneously acquired at 1 s intervals, however, for clarity, error bars (sem) are plotted only every 10sBack to article page