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Fig. 7 | BMC Physiology

Fig. 7

From: Ryanodine-induced vasoconstriction of the gerbil spiral modiolar artery depends on the Ca2+ sensitivity but not on Ca2+ sparks or BK channels

Fig. 7

Inhibition of Ca2+ sparks with ryanodine increases global Ca2+ and constricts SMA at 60 cmH2O following an increase in Ca2+ sensitivity by endothelin-1. a Representative recordings of cytosolic Ca2+ changes from single smooth muscle cells from a SMA loaded with fluorescent dye fluo4 and pressurized to 60 cmH2O in response to 10 μM ryanodine after treatment with 1 nM endothelin-1 (ET-1). b Average of normalized traces of cytosolic Ca2+ changes at 60 cmH2O (64 cells from 7 arteries). Traces in a and b were normalized as described in Methods. c Average trace of corresponding changes in vascular diameter of SMA pressurized at 60 cmH2O (15 arteries). Diameter changes were normalized to the average of values recorded between 30–60 s (value indicated by ‘a’ was set to 1). Ca2+ and diameter data were simultaneously acquired at 1 s intervals, however, for clarity, error bars (sem) are plotted only every 10 s

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