Fig. 2

NF-κB siRNA administration decreased NF-kB transcript and protein levels in lung tissues of rats with ALI. a qRT-PCR was used to compare the levels of NF-κB mRNA transcription between LPS + scramble group and LPS + siRNA group. The amplification of GAPDH was used as internal control. Data were expressed as mean ± S.D. from three individual experiments. *P < 0.05 vs. LPS+. b Rats were treated with siRNA specific to NF-κB for one or eight hours, lung tissue was collected and processed by western blot analysis to determine the levels of NF-κB p65 expression after one or eight hours