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Fig. 6 | BMC Physiology

Fig. 6

From: Small interfering RNA targeting NF-κB attenuates lipopolysaccharide-induced acute lung injury in rats

Fig. 6

Specific targeting of NF-κB ablates TNF-α transcript and protein levels in lung tissue. Rats were treated with siRNA specific to NF-κB and TNF-α transcript levels were assessed at 1 and 8 h by qRT-PCR and compared between LPS + scramble group and LPS + siRNA group (a). The amplification of GAPDH was used as internal control. Data were expressed as mean ± S.D. from three individual experiments. #P < 0.05 vs. scramble group. Rats were treated with siRNA specific to NF-κB and TNF-α levels were assessed at one and eight hrs by standard ELISA (b). Data were expressed as mean ± S.D. (n = 12). *P < 0.05 vs. saline + DEPC;#P < 0.05 vs. LPS + DEPC;P < 0.05 vs. LPS + scramble

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