Fig. 3

Localization of selected claudin isoforms (see text) in the cochlea. a The cochlea was dissected into three parts (lateral wall, medial region and outer bone). Transcript expression is shown for cldn-5, − 6, − 7, −10v1, −10v2, − 13, − 15, −19v1, −19v2, − 20, − 22, − 23 (n = 3) in each fraction. Ages of samples are between P18 and P32 (days), mean: 22.6. Asterisks indicate significant difference between tissues. Claudins in the lateral wall and medial fractions that were highly expressed (claudin mRNA/18S > 4.5; dashed line) in this experimental series were analyzed in the more-finely separated tissues of the following series. b The lateral and medial fractions were each subdivided into two smaller fractions in order to obtain finer resolution of location (n = 6). Lateral wall: stria vascularis and spiral ligament; Medial region: organ of Corti and modiolus. Ages of samples are between P19 and P28 (days), mean: 22.0. Asterisks indicate significant difference (P < 0.05) using the two-way analysis of variance as indicated by brackets; *, significant. Non-significant comparisons are not shown and comparisons other than stria vascularis – spiral ligament and organ of Corti – modiolus are given in the Additional file 1. Error bars, Standard Deviation