Fig. 8
From: Deficiency of the BMP Type I receptor ALK3 partly protects mice from anemia of inflammation
Hepatic STAT3 and SMAD1/5/8 protein expression in Alk3fl/fl; Alb-Cre and Alk3fl/fl mice. Representative western blots (a) and quantitative analyses (b) of phospho-STAT3 and total-STAT3 from Alk3fl/fl and Alk3fl/fl; Alb-Cre mice 14 days after heat-killed Brucella abortus (BA) or saline injection. Two isoforms of phospho-STAT3 α and β (79/86 kDa) exist. Loading control is indicated by total STAT3 protein levels. c SMAD1/5/8 activation 14d after BA administration in Alk3fl/fl (Cre-) and hepatocyte-specific Alk3 deficient (Alk3fl/fl; Alb-Cre) (Cre+) mice fed an iron deficient diet. Western blots from liver proteins analysed with phosphorylated SMAD1/5/8, total SMAD1, and α-tubulin antibodies. Arrows indicate specific pSMAD signal. d Densitometric analyses of the ratio of SMAD1/5/8 to SMAD1