Claudin expression during early postnatal development of the murine cochlea

Background Claudins are major components of tight junctions, which form the paracellular barrier between the cochlear luminal and abluminal fluid compartments that supports the large transepithelial voltage difference and the large concentration differences of K+, Na+ and Ca2+ needed for normal cochlear function. Claudins are a family of more than 20 subtypes, but our knowledge about expression and localization of each subtype in the cochlea is limited. Results We examined by quantitative RT-PCR the expression of the mRNA of 24 claudin isoforms in mouse cochlea during postnatal development and localized the expression in separated fractions of the cochlea. Transcripts of 21 claudin isoforms were detected at all ages, while 3 isoforms (Cldn-16, − 17 and − 18) were not detected. Claudins that increased expression during development include Cldn-9, − 13, − 14, − 15, and -19v2, while Cldn-6 decreased. Those that do not change expression level during postnatal development include Cldn-1, − 2, − 3, − 4, − 5, − 7, − 8, −10v1, −10v2, − 11, − 12, −19v1, − 20, − 22, and − 23. Our investigation revealed unique localization of some claudins. In particular, Cldn-13 expression rapidly increases during early development and is mainly expressed in bone but only minimally in the lateral wall (including stria vascularis) and in the medial region (including the organ of Corti). No statistically significant changes in expression of Cldn-11, − 13, or − 14 were found in the cochlea of Slc26a4−/− mice compared to Slc26a4+/− mice. Conclusions We demonstrated developmental patterns of claudin isoform transcript expression in the murine cochlea. Most of the claudins were associated with stria vascularis and organ of Corti, tissue fractions rich in tight junctions. However, this study suggests a novel function of Cldn-13 in the cochlea, which may be linked to cochlear bone marrow maturation. Electronic supplementary material The online version of this article (10.1186/s12899-018-0035-1) contains supplementary material, which is available to authorized users.


Background
Tight junctions are structures consisting of proteins that join epithelial and endothelial cells to form continuous sheets and tubules which separate two liquid compartments. They consist of claudins [1], occludins [2] and other proteins that form a band-like network known as tight junction strands. These junctions are known to perform several functions (barrier, pore and fence), and are composed of several types of proteins: transmembrane (e.g., claudins and occludin), cytoplasmic, signaling and adapter links to the cytoskeleton [1,3]. Barrier function refers to the restriction of paracellular movement of fluid constituents between the two fluid compartments that are separated by the cell layer. Pore function refers to the selective permeability of the paracellular barrier to those solutes that can pass between the fluid compartments. The fence function refers to the restriction of lateral movement of membrane proteins and lipids within the face of the plasma membrane, which retains the separate physiological functions of the luminal and abluminal cell membranes that are necessary to carry out vectorial transport of solutes and water.
Claudins are a family of more than 20 subtypes [1]. The specific isoforms of claudin included in a tight junction are the primary determinant of paracellular permeability [3]. Common structures of the claudin family include four transmembrane domains and two extracellular loops (Fig. 1). It is thought that charged amino acids in the first extracellular loop define the permeability of tight junctions, while the second extracellular loop contributes toward adhesion of the apposed cell membranes (Fig. 1) [1,4]. Multiple claudin isoforms are usually co-expressed in one tissue and their mixing ratio determines the permeability properties of the tight junction in that tissue [1,5]. Claudins are regulated in their expression, same-cell and neighboring-cell interactions, modulations and degradation by numerous separate pathways and networks [3].
Claudins are known to be critical for normal hearing [6,7]. A major driving force for the ionic currents underlying the cellular transduction of sound into corresponding electrical signals to hearing centers in the brain is the endocochlear potential, the transepithelial voltage across the inner ear epithelium [8]. This voltage is generated within the multi-layered stria vascularis in the cochlear lateral wall and originates as a potential difference across the basal cell layer of the stria between the intrastrial fluid space and the perilymph pervading the fibrous spiral ligament [8]. This potential difference across the basal cell layer is supported by the barrier function of the highly dense tight junctions between the basal cells, as confirmed by the reduction of endocochlear potential and the resulting deafness in adult Cldn-11 knockout mice [6]. In contrast to this pathology of stria vascularis, mutation of Cldn-14 led to degeneration of a different cochlear structure, the sensory organ of Corti, and was associated with the human hereditary deafness DFNB29 [9,10]. It is to be expected that mutations of other claudin isoforms in the cochlea could lead to impaired hearing. In addition to these examples of claudin isoform localization and expression, three other groups of investigators have reported expression of claudins in the cochlea [11][12][13]. Kitajiri and colleagues [12] examined Cldn-1 to Cldn-18 using immunohistochemistry, but their study was limited by a lack of antibodies for some claudins. We localized in the current study transcript expression of most of the claudin isoforms in multiple tissue fractions of the cochlea, including the outer layer of cochlear bone.
One of the most common hereditary deafness genes is pendrin (SLC26A4), which has been surprisingly shown to exert its strongest effects on cochlear function by expression in the endolymphatic sac during early development [14]. Lack of pendrin expression was found to be accompanied by delays in cochlear bone development and in expression of other genes due to an apparent local hypothyroidism [15]. It therefore was of interest to determine whether the expression of Cldn-13, observed in the present study to be predominantly expressed in the outer bone fraction, would be altered by deletion of the Slc26a4 gene in the mouse model.
The aim in the present study was to determine a) expression of claudin transcripts during early development, b) localization of the claudin isoforms among the cochlear regions and c) the potential effects of Slc26a4 knockout on claudin isoform expression.

Methods
Slc26a4 +/− and Slc26a4 −/− mice were obtained from a colony at Kansas State university and the heterozygous mice served as controls. Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.). Temporal bones were removed from both male and female mice and whole cochleae were collected from age-sex matched littermates of Slc26a4 +/− and Slc26a4 −/− . Expression of claudins was determined on RNA isolated from a) whole cochlea, b) cochlear lateral wall tissues, c) cochlear medial fraction tissues, and d) outer cochlear bone. Lateral wall tissues were further microdissected into spiral ligament and stria vascularis fractions, while the medial fraction was further microdissected into organ of Corti and modiolus fractions. All procedures involving animals were approved by the Institutional Animal Care and Use Committee of Kansas State University (protocol 2925).
Total RNA was isolated from these tissues using the RNeasy Micro Kit (Qiagen, Valencia, CA; Cat #7400) and care was taken that RNA was extracted from all cell types. Recombinant bovine DNase I, Grade 1 (Roche Diagnostics Corp, Indianapolis, IN; catalog # 04536282001) was used to remove residual DNA. The quality and quantity of 18S rRNA were determined by using the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA; catalog # 5067-1511) with a Bioanalyzer (Agilent Technologies; Model 2100) and a spectrophotometer (Thermo Scientific, Wilmington, DE; NanoDrop 8000). The amount of RNA in each sample was calculated as the average of the results of the Bioanalyzer and NanoDrop assays.
Primer pairs for mouse Cldn-1 to − 23 (excluding Cldn-21) were designed using software Primer3 (http:// primer3.sourceforge.net/). The sequences of primers are documented in Table 1 and were validated with RNA from positive control tissues (tibia, liver, lung, intestine, kidney, stomach, skin, brain). mRNA expression was measured by quantitative RT-PCR using a Bio-Rad icycler iQ thermocycler and QuantiTect SYBR Green RT-PCR Kit (Qiagen; catalog # 204243). Claudin mRNA was normalized against 18S. The calculation method has been described previously [16]. Melting curve measurements were made with the Bio-Rad thermocycler and the PCR product size was measured by using a DNA assay (Agilent DNA 1000 Kit; catalog #5067-1504) on the Agilent Bioanalyzer to exclude the detection of nonspecific PCR products. This method yields quantitative measures of claudin isoform transcript expression that can be compared within each isoform; however, comparisons between and among isoforms are not quantitative in these experiments due to undetermined efficiencies in the RT step that vary for each primer pair [16].
Data are given as means ± standard deviation (SD) or ± standard error of the mean (SEM), as reported in Results. N values refer to the number of cochleae (Figs. 2 and 4) or to the number of isolated tissues ( Fig. 3a and b) which are the same as the numbers of RT-PCR reactions analyzed. A one-way analysis of variance (ANOVA) with Holm-Sidak method post-test (Figs. 2 and 3) or a two-way ANOVA which tested for statistical significance of interaction between age and genotype. Since no statistically significant interaction was found for the 3 transcripts tested in the experiments shown in Fig. 4, individual paired differences were not assessed. P values of < 0.05 were considered as significant differences; analyses were performed with SigmaStat for Windows Version 4.0 software.

Results and discussion
We first determined the transcript expression level of 24 claudin isoforms in the whole cochlea of normal (Slc26a4 +/− ) mice at three different ages after birth: P2 and P6 before the onset of endocochlear potential generation and hearing in mice, and at P15, after acquisition of hearing. Transcripts of 21 claudin isoforms were detected at all ages, while 3 isoforms (Cldn-16, − 17 and − 18) were not detected (Fig. 2). The permeability properties of several isoforms have been unambiguously determined [17,18] and are shown in (Fig. 2), as described in the figure legend. Cldn-10 [19] and Cldn-19 were each determined for two splice variants, v1 and v2 (Table 1).
Six cochlear claudin isoforms increase with development at P6 and/or P15: Cldn-9, − 13, − 14, − 15, and -19v2. By contrast, Cldn-6 expression decreases with development. Cochlear claudins that do not change significantly with development include: Cldn-1, − 2, − 3, − 4, − 5, − 7, − 8, −10v1, −10v2, − 11, − 12, −19v1, − 20, − 22, and − 23. As described above, endocochlear potential normally develops between P6 and P15. So genes that change their expression in this period might be involved in establishment of the special properties of the paracellular barrier of the epithelial cells that border the endolymph, and thereby provide the resistive barrier that supports the large endocochlear potential. Cldn-19v2 appeared to increase expression only transiently during this period. The post-natal changes in expression of multiple claudin isoforms are consistent with the likely presence of factors that regulate claudin expression during development. Most striking of all, Cldn-13 shows a remarkably large increase in cochlear expression compared to the others. Previously, Abuazza et al. [20] reported maturational decrease of Cldn-6, − 9 and − 13 transcripts and of paracellular protein in several segments of the mouse kidney. They suggested these changes may contribute to developmental changes in the paracellular permeability of kidney tubules. In our study of the cochlea, Cldn-6 undergoes developmental decrease in transcript expression from P2 to P6, and further from P6 to P15, as in the kidney (Fig. 2). By contrast to the kidney, Cldn-9 and -13 transcripts increased from P2 to P6, and further from P6 to P15 (Fig. 2).
The cochlear tissues expressing these claudins were resolved in two subsequent experimental series. In the first series (Fig. 3a), cochleae of adult (P18-P32, mean P22.6) Slc26a4 +/− mice were subdivided into three fractions: 1) lateral wall (exclusive of outer bone), 2) medial region, and 3) outer bone. These fractions were assayed for 12 claudin isoforms: Cldn-5,   [22] and Cldn-13 was identified in a stress induced erythropoiesis pathway that is mainly expressed in tissues associated with haematopoietic function [23]. It is therefore likely that expression of Cldn-13 in cochlear outer bone might originate from the associated bone marrow, which develops during the   [17]. Asterisks indicate significant difference (P < 0.05) between bars embraced by brackets using one-way ANOVA and the Holm-Sidek post-test. The absence of brackets and asterisks indicates differences are not significant . Error bars, Standard Deviation. The individual descriptive statistics are derived from n cochleae, as indicated on the graph early postnatal period [15,24]. In support of this proposition, it was found that Slc26a4 −/− mice exhibit delayed bone marrow maturation between P6 and P15 [24]. Mouse Cldn-13 does not have a human homolog [3].
In the second series, cochleae of adult (P19-P28, mean P22.0) Slc26a4 +/− mice were subdivided further into four micro-dissected fractions: 1) the stria vascularis and 2) spiral ligament fractions were separated from the lateral wall; 3) the organ of Corti and the 4) modiolus were separated from the medial structures. Claudins in the lateral wall and medial fractions that gave high expression signals (claudin mRNA/18S > 4.5) in the first experimental series (Fig. 3a) were analyzed in the more-finely separated tissues of the second series (Fig. 3b). The epithelial fractions (stria vascularis and organ of Corti) were found to express Cldn-7 more strongly than their respective primarily non-epithelial fractions, spiral ligament (fibrocytes) and modiolus (neurons). By contrast, the other six isoforms did not show statistically significant differences between the epithelial fractions and their respective adjacent non-epithelial fractions. Non-significant comparisons are not shown in Fig. 3b and comparisons other than stria vascularisspiral ligament and organ of Cortimodiolus are given in the Additional file 1.
Three claudins were selected to investigate the possible effect of Slc26a4 gene deletion on inner ear developmental expression of claudins. Developmental expression of the three isoforms demonstrated a dramatic postnatal increase in Cldn-13 that was not characteristic of the other two claudins, consistent with the notion that Cldn-13 is not regulated by a mechanism common to the claudins highly expressed in the epithelial tissues. We examined RNA from whole cochleae from age-and sex-matched littermates of Slc26a4 +/− and Slc26a4 −/− and analyzed by two-way ANOVA 1) Cldn-11, which is expressed in basal cells of stria vascularis [12], and whose deletion in mice causes hearing loss, 2) Cldn-13, which is expressed in cochlear outer bone (this report), and 3) Cldn-14, which is expressed in organ of Corti and is responsible for human hereditary deafness DFNB29. The results of analysis (Fig. 4) showed no statistically significant interaction between age and genotype in all three genes and no further comparisons of individual paired genotypes were made. Ages of samples are between P18 and P32 (days), mean: 22.6. Asterisks indicate significant difference between tissues. Claudins in the lateral wall and medial fractions that were highly expressed (claudin mRNA/18S > 4.5; dashed line) in this experimental series were analyzed in the more-finely separated tissues of the following series. b The lateral and medial fractions were each subdivided into two smaller fractions in order to obtain finer resolution of location (n = 6). Lateral wall: stria vascularis and spiral ligament; Medial region: organ of Corti and modiolus. Ages of samples are between P19 and P28 (days), mean: 22.0. Asterisks indicate significant difference (P < 0.05) using the two-way analysis of variance as indicated by brackets; *, significant. Non-significant comparisons are not shown and comparisons other than stria vascularisspiral ligament and organ of Cortimodiolus are given in the Additional file 1. Error bars, Standard Deviation "Cldn-21" was not included in this study. The nomenclature has varied and developed since 2001 and was not identified in mouse at the time of this study [25]. The mouse gene currently accepted as Cldn-21 [25,26] has been heterologously expressed in MDCK epithelial cell cultures, immunolocalized to sites that also express the tightjunction protein occludin, and was shown to participate in a Na + -selective paracellular transport pathway [26].
Some of our data differ from previous observations: expression of Cldn-5, − 6 and − 15 was not detected by Kitajiri et al. [12], but were observed in our experiments. In kidney, Cldn-5 and -15 are expressed in endothelial cells, not epithelial cells [27]. By contrast, Kitajiri et al. [12] reported that there had been no expression of Cldn-5 and Cldn-15 in stria vascularis nor spiral ligament, both highly vascularized tissues. We found Cldn-6 expression, but it gradually decreased during early development. Consistent with our observation, Kitajiri et al. [12] did not see any expression of Cldn-6 in the adult cochlea.

Conclusions
We analyzed 24 claudins in structures of the inner ear. Previous studies did not show the presence and localization of Cldn-7, Cldn-13, Cldn-19 to − 23 in the cochlea, but the results of our study showed regional localization of transcripts of these genes in the cochlea and developmental changes in two of them. We observed that Cldn-13 is expressed in bone and that its expression increased rapidly during early postnatal development. Most of the claudins were expressed in stria vascularis and organ of Corti, tissue fractions rich in tight junctions. However, this study suggests a novel function of Cldn-13 in the cochlea, which may be linked to cochlear bone marrow maturation.