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Figure 4 | BMC Physiology

Figure 4

From: Bridging the phenotypic gap: Real-time assessment of mitochondrial function and metabolism of the nematode Caenorhabditis elegans

Figure 4

Bioluminescence response following RNAi of respiratory chain genes. Decrease in Luminescence was consistent with reported changes in in vitro ATP levels. Animals [glp-4(bn2); feIs5] were exposed to dsRNA from the L1 larval stage, for 4 days, at 25°C. Luminescence was normalised to average length of worms. Differences in intensity of fluorescence were only evident when gfp was targeted by RNAi. Luciferase expression is also lowered with the gfp RNAi clone, as the luc+ and gfp genes are fused. Error bars show the SEM. The pooled number of worms was 480 (± 26) per tested condition.

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