Comparison of EB uptake and LDH release in BAECs. EB uptake (red lines) was determined in the absence and presence of 0.3 nM MTX as indicated to the right of each trace. The solid lines are mean values from 3 experiments; for clarity ± SE values are only shown at selected time points, i.e. every 20 sec. In paired experiments, LDH release (black symbols) was monitored as described in Material and Methods. The values shown represent the mean ± SE LDH release determined in the presence (black circles) or absence (black diamonds) of 0.3 nM MTX. Note that LDH release in the absence of MTX was only determined at time zero and at time 17 min.