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Table 1 Boltzmann parameters of Ca2+ conductance, charge movements and Ca2+ transients of control, γ1 het and γ1 null myotubes.

From: Modulation of L-type Ca2+ current but not activation of Ca2+ release by the gamma1 subunit of the dihydropyridine receptor of skeletal muscle

CELL TYPE

G-V

Q-V

 

Gmax (pS/pF)

V1/2 (mV)

k (mV)

Qmax (fC/pF)

V1/2 (mV)

k (mV)

Control, +/+

131 ± 6.2

14.7 ± 2.1

4.2 ± 0.4

5.3 ± 0.4

11.4 ± 2.5

15.4 ± 1.1

(n)

(8)

  

(8)

  

γ 1 het, +/-

161 ± 11.5a

14.3 ± 2.8

5 ± 0.8

5.3 ± 0.3

14.4 ± 0.6

14.7 ± 2.0

(n)

(8)

  

(4)

  

γ 1 null, -/-

170 ± 9.6b

10.9 ± 0.9

4.5 ± 0.4

5.3 ± 0.9

17.8 ± 1.9

15.8 ± 2.2

(n)

(12)

  

(8)

  
 

ΔF/Fo-V

 

Inactivation

 

ΔF/Fo(max)

V1/2 (mV)

k (mV)

 

V1/2 (mV)

k (mV)

Control, +/+

3.9 ± 0.3

4.6 ± 1.2

10.2 ± 1.3

 

-4.6 ± 3.6

7.3 ± 0.2

(n)

(6)

   

(6)

 

γ 1 het, +/-

3.3 ± 0.5

8.2 ± 3.0

10.1 ± 2.0

 

8.1 ± 2.3c

8.0 ± 0.6

(n)

(4)

   

(8)

 

γ 1 null, -/-

3.7 ± 0.4

7.3 ± 1.6

9.0 ± 1.1

 

8.9 ± 0.8d

8.1 ± 0.8

(n)

(6)

   

(8)

 
  1. Entries (mean ± SEM) correspond to Boltzmann parameters fitted to each cell (number of cells n, in parenthesis). The only statistically significant differences (p<0.05) were for either γ1 het or γ1 null cells compared to control cells as indicated. The t-test p values were 0.004, 0.009, 0.011, 0.009 for a-d, respectively.